Harnessing bioengineered microbes as a versatile platform for space nutrition – Nature Communications

  • Berliner, A. J. et al. Towards a biomanufactory on Mars. Front. Astron Space Sci. 8, 711550 (2021). This article presents a comprehensive study toward developing sustainable biomanufacturing for extended human operations on Mars.

    Article 

    Google Scholar
     

  • Berliner, A. J. et al. Space bioprocess engineering on the horizon. Commun. Eng. 1, 13 (2022).

  • Douglas, G. L., Zwart, S. R. & Smith, S. M. Space food for thought: challenges and considerations for food and nutrition on exploration missions. J. Nutr. 150, 2242–2244 (2020).

    Article 
    PubMed 

    Google Scholar
     

  • Montague, M. et al. The role of synthetic biology for in situ resource utilization (ISRU). Astrobiology 12, 1135–1142 (2012).

    Article 
    ADS 
    PubMed 

    Google Scholar
     

  • Nangle, S. N. et al. The case for biotech on Mars. Nat. Biotechnol. 38, 401–407 (2020).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Llorente, B., Williams, T. C. & Goold, H. D. The multiplanetary future of plant synthetic biology. Genes 9, 348 (2018). The authors discuss using synthetic biology approaches to take full advantage of plants to contribute to supporting human ventures off-Earth.

    Article 
    PubMed Central 

    Google Scholar
     

  • Menezes, A. A., Cumbers, J., Hogan, J. A. & Arkin, A. P. Towards synthetic biological approaches to resource utilization on space missions. J. R. Soc. Interface 12, 20140715 (2015).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Way, J. C., Silver, P. A. & Howard, R. J. Sun-driven microbial synthesis of chemicals in space. Int. J. Astrobiol. 10, 359–364 (2011).

    Article 
    ADS 
    CAS 

    Google Scholar
     

  • Cannon, K. M. & Britt, D. T. Feeding one million people on Mars. N. Space 7, 245–254 (2019).

    Article 
    ADS 

    Google Scholar
     

  • Hader, D. P. On the way to Mars-flagellated algae in bioregenerative life support systems under microgravity conditions. Front. Plant Sci. 10, 1621 (2019).

    Article 
    PubMed 

    Google Scholar
     

  • Choi, K. R., Yu, H. E. & Lee, S. Y. Microbial food: microorganisms repurposed for our food. Micro. Biotechnol. 15, 18–25 (2022). This article discusses repurposing microorganisms as food, comparing microbial-, animal-, and plant-derived biomass production’s environmental impact and nutritional properties.

    Article 

    Google Scholar
     

  • Sun, L., Xin, F. & Alper, H. S. Bio-synthesis of food additives and colorants-a growing trend in future food. Biotechnol. Adv. 47, 107694 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Linder, T. Making the case for edible microorganisms as an integral part of a more sustainable and resilient food production system. Food Security 11, 265–278 (2019). This review summarizes microbial-based food’s challenges and potential impacts in addressing environmental sustainability and food security. Basic nutritional properties of microbial food products are also compared to other food products.

    Article 

    Google Scholar
     

  • Samuel, D. Investigation of ancient egyptian baking and brewing methods by correlative microscopy. Science 273, 488–490 (1996).

    Article 
    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Onofre, S. B., Bertoldo, I. C., Abatti, D. & Refosco, D. Chemical composition of the biomass of Saccharomyces cerevisiae – (Meyen ex E. C. Hansen, 1883) yeast obtained from the beer manufacturing process. Int. J. Environ. Agric Biotechnol. 2, 558–562 (2017).


    Google Scholar
     

  • Nielsen, J. Yeast systems biology: model organism and cell factory. Biotechnol. J. 14, e1800421 (2019).

    Article 
    PubMed 

    Google Scholar
     

  • Purevdorj-Gage, B., Sheehan, K. B. & Hyman, L. E. Effects of low-shear modeled microgravity on cell function, gene expression, and phenotype in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 72, 4569–457 (2006).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Bell, P. J. L. et al. An electro-microbial process to uncouple food production from photosynthesis for application in space exploration. Life 12, 1002 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Stern, J. C. et al. Evidence for indigenous nitrogen in sedimentary and aeolian deposits from the Curiosity rover investigations at Gale crater, Mars. Proc. Natl Acad. Sci. USA 112, 4245–4250 (2015).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Meerman, R. & Brown, A. J. When somebody loses weight, where does the fat go? BMJ 349, g7257 (2014).

      'Buransh flower' can cure many diseases including cancer, know why it is so beneficial?

    Article 
    PubMed 

    Google Scholar
     

  • Dai, Z. et al. Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae. Bioresour. Technol. 245, 1407–1412 (2017).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Espinosa, M. I. et al. Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae. Nat. Commun. 11, 5564 (2020). Methylotrophic metabolism enables growth on methanol, a one-carbon alternative to sugar fermentation. Here the authors use adaptive laboratory evolution to uncover native methylotrophy capacity in the yeast Saccharomyces cerevisiae.

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Averesch, N. J. Choice of microbial system for in-situ resource utilization on Mars. Front. Astron Space Sci. 8, 700370 (2021).

    Article 

    Google Scholar
     

  • Somoza-Tornos, A., Guerra, O. J., Crow, A. M., Smith, W. A. & Hodge, B. M. Process modeling, techno-economic assessment, and life cycle assessment of the electrochemical reduction of CO2: a review. iScience 24, 102813 (2021).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Looser, V. et al. Cultivation strategies to enhance productivity of Pichia pastoris: a review. Biotechnol. Adv. 33, 1177–1193 (2015).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Verduyn, C. Physiology of yeasts in relation to biomass yields. Antonie Leeuwenhoek 60, 325–353 (1991).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Verseux, C. et al. Sustainable life support on Mars—the potential roles of cyanobacteria. Int. J. Astrobiol. 15, 65–92 (2016).

    Article 
    ADS 
    CAS 

    Google Scholar
     

  • Verseux, C. et al. A Low-Pressure, N2/CO2 atmosphere is suitable for cyanobacterium-based life-support systems on Mars. Front. Microbiol. 12, 611798 (2021).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Ducat, D. C., Avelar-Rivas, J. A., Way, J. C. & Silver, P. A. Rerouting carbon flux to enhance photosynthetic productivity. Appl. Environ. Microbiol. 78, 2660–2668 (2012).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Guadalupe-Medina, V. et al. Carbon dioxide fixation by Calvin-Cycle enzymes improves ethanol yield in yeast. Biotechnol. Biofuels 6, 125 (2013).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Papapetridis, I. et al. Optimizing anaerobic growth rate and fermentation kinetics in Saccharomyces cerevisiae strains expressing Calvin-cycle enzymes for improved ethanol yield. Biotechnol. Biofuels 11, 17 (2018).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Gassler, T. et al. The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2. Nat. Biotechnol. 38, 210–216 (2020). The authors engineered the metabolism of the yeast Pichia pastoris to enable it to grow as an autotrophic organism using CO2 as a sole carbon source.

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Gleizer, S. et al. Conversion of Escherichia coli to generate all biomass carbon from CO2. Cell 179, 1255–1263 (2019).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Fabarius, J. T., Wegat, V., Roth, A. & Sieber, V. Synthetic methylotrophy in yeasts: towards a circular bioeconomy. Trends Biotechnol. 39, 348–358 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Gonzalez de la Cruz, J., Machens, F., Messerschmidt, K. & Bar-Even, A. Core catalysis of the reductive glycine pathway demonstrated in yeast. ACS Synth. Biol. 8, 911–917 (2019).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Espinosa, M. I., Williams, T. C., Pretorius, I. S. & Paulsen, I. T. Benchmarking two Saccharomyces cerevisiae laboratory strains for growth and transcriptional response to methanol. Synth. Syst. Biotechnol. 4, 180–188 (2019).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Marcellin, E., Angenent, L. T., Nielsen, L. K. & Molitor, B. Recycling carbon for sustainable protein production using gas fermentation. Curr. Opin. Biotechnol. 76, 102723 (2022).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Godard, P. et al. Effect of 21 different nitrogen sources on global gene expression in the yeast Saccharomyces cerevisiae. Mol. Cell Biol. 27, 3065–3086 (2007).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

      हिस्टेरेक्टॉमी के लिए दिल्ली के अच्छे अस्पताल - Best Hindi Health Tips (हेल्थ टिप्स), Healthcare Blog - News | GoMedii

  • Brabender, M., Hussain, M. S., Rodriguez, G. & Blenner, M. A. Urea and urine are a viable and cost-effective nitrogen source for Yarrowia lipolytica biomass and lipid accumulation. Appl. Microbiol. Biotechnol. 102, 2313–2322 (2018).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Santa Maria, S. R., Marina, D. B., Tieze, S. M., Liddell, L. C. & Bhattacharya, S. BioSentinel: long-term Saccharomyces cerevisiae preservation for a deep space biosensor mission. Astrobiology 20, 1–14 (2020).

  • Postma, E. D. et al. Modular, synthetic chromosomes as new tools for large scale engineering of metabolism. Metab. Eng. 72, 1–13 (2022). This article describes the development of synthetic neochromosomes with modular design as platforms for reprogramming yeast metabolism and installing new functionalities.

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Kutyna, D. R. et al. Construction of a synthetic Saccharomyces cerevisiae pan-genome neo-chromosome. Nat. Commun. 13, 3628 (2022). This work demonstrates the concept of using synthetic neochromosomes to provide phenotypic plasticity to yeast, including expanding the range of utilizable carbon sources.

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Beekwilder, J. et al. Polycistronic expression of a beta-carotene biosynthetic pathway in Saccharomyces cerevisiae coupled to beta-ionone production. J. Biotechnol. 192 Pt B, 383–392 (2014).

    Article 
    PubMed 

    Google Scholar
     

  • Majer, E., Llorente, B., Rodriguez-Concepcion, M. & Daros, J. A. Rewiring carotenoid biosynthesis in plants using a viral vector. Sci. Rep. 7, 41645 (2017).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Dixon, T. A., Williams, T. C. & Pretorius, I. S. Sensing the future of bio-informational engineering. Nat. Commun. 12, 388 (2021).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Walker, R. S. K. & Pretorius, I. S. Synthetic biology for the engineering of complex wine yeast communities. Nat. Food 3, 249–254 (2022).

    Article 
    CAS 

    Google Scholar
     

  • Besong, S., Jackson, J. A., Hicks, C. L. & Hemken, R. W. Effects of a supplemental liquid yeast product on feed intake, ruminal profiles, and yield, composition, and organoleptic characteristics of milk from lactating Holstein cows. J. Dairy Sci. 79, 1654–1658 (1996).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Yu, T. et al. Reprogramming yeast metabolism from alcoholic fermentation to lipogenesis. Cell 174, 1549–1558.e1514 (2018).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Yazawa, H., Iwahashi, H., Kamisaka, Y., Kimura, K. & Uemura, H. Improvement of polyunsaturated fatty acids synthesis by the coexpression of CYB5 with desaturase genes in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 87, 2185–2193 (2010).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Tavares, S. et al. Metabolic engineering of Saccharomyces cerevisiae for production of Eicosapentaenoic Acid, using a novel Delta 5-Desaturase from Paramecium tetraurelia. Appl. Environ. Microbiol. 77, 1854–1861 (2011).

    Article 
    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Qiu, X., Hong, H. & MacKenzie, S. L. Identification of a Delta 4 fatty acid desaturase from Thraustochytrium sp. involved in the biosynthesis of docosahexanoic acid by heterologous expression in Saccharomyces cerevisiae and Brassica juncea. J. Biol. Chem. 276, 31561–31566 (2001).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Larroude, M., Rossignol, T., Nicaud, J. M. & Ledesma-Amaro, R. Synthetic biology tools for engineering Yarrowia lipolytica. Biotechnol. Adv. 36, 2150–2164 (2018).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Beopoulos, A. et al. Control of lipid accumulation in the yeast Yarrowia lipolytica. Appl. Environ. Microbiol. 74, 7779–7789 (2008).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Yuan, S. F. & Alper, H. S. Metabolic engineering of microbial cell factories for production of nutraceuticals. Micro. Cell Fact. 18, 46 (2019).

    Article 

    Google Scholar
     

  • Sun, L., Kwak, S. & Jin, Y. S. Vitamin A Production by engineered Saccharomyces cerevisiae from xylose via two-phase in situ extraction. ACS Synth. Biol. 8, 2131–2140 (2019).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Branduardi, P. et al. Biosynthesis of vitamin C by yeast leads to increased stress resistance. PLoS ONE 2, e1092 (2007).

    Article 
    ADS 
    PubMed 
    PubMed Central 

    Google Scholar
     

      Advice | Ask a Doctor: Are salads actually good for you?

  • Guo, X. J. et al. Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction. Biotechnol. Biofuels 11, 192 (2018).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Wang, Y., Liu, L., Jin, Z. & Zhang, D. Microbial cell factories for green production of vitamins. Front. Bioeng. Biotechnol. 9, 66156 (2021).


    Google Scholar
     

  • van Wyk, N., Kroukamp, H. & Pretorius, I. S. The smell of synthetic biology: engineering strategies for aroma compound production in yeast. Fermentation 4, 54 (2018).

    Article 

    Google Scholar
     

  • Kallscheuer, N., Classen, T., Drepper, T. & Marienhagen, J. Production of plant metabolites with applications in the food industry using engineered microorganisms. Curr. Opin. Biotechnol. 56, 7–17 (2019).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Denby, C. M. et al. Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer. Nat. Commun. 9, 965 (2018). A neat example of how metabolic engineering can enable the production of plant-derived flavor molecules in yeast.

    Article 
    ADS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Lee, D., Lloyd, N. D., Pretorius, I. S. & Borneman, A. R. Heterologous production of raspberry ketone in the wine yeast Saccharomyces cerevisiae via pathway engineering and synthetic enzyme fusion. Micro. Cell Fact. 15, 49 (2016).

    Article 

    Google Scholar
     

  • Hansen, E. H. et al. De novo biosynthesis of vanillin in fission yeast (Schizosaccharomyces pombe) and baker’s yeast (Saccharomyces cerevisiae). Appl. Environ. Microbiol. 75, 2765–2774 (2009).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Shankar, S. & Hoyt, M. A. United States Patent and Trademark Office. Expression constructs and methods of genetically engineering methylotrophic yeast, (ed. USPTO). USA patent (2020).

  • Dance, A. Engineering the animal out of animal products. Nat. Biotechnol. 35, 704–707 (2017).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Buldum, G., Bismarck, A. & Mantalaris, A. Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli. Bioprocess Biosyst. Eng. 41, 265–279 (2018).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Pfister, B. et al. Recreating the synthesis of starch granules in yeast. Elife 5, e15552 (2016).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Sen, T., Barrow, C. J. & Deshmukh, S. K. Microbial pigments in the food industry-challenges and the way forward. Front. Nutr. 6, 7 (2019).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Keppler-Ross, S., Noffz, C. & Dean, N. A new purple fluorescent color marker for genetic studies in Saccharomyces cerevisiae and Candida albicans. Genetics 179, 705–710 (2008).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Mitchell, L. A. et al. Versatile genetic assembly system (VEGAS) to assemble pathways for expression in S. cerevisiae. Nucleic Acids Res. 43, 6620–6630 (2015).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Wehrs, M. et al. Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae. Micro. Cell Fact. 17, 193 (2018).

    Article 
    CAS 

    Google Scholar
     

  • DeLoache, W. C., Russ, Z. N. & Dueber, J. E. Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways. Nat. Commun. 7, 11152 (2016).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Liljeruhm, J. et al. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. J. Biol. Eng. 12, 8 (2018).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Sun, J., Peng, Z., Yan, L., Fuh, J. Y. & Hong, G. S. 3D food printing—An innovative way of mass customization in food fabrication. Int. J. Bioprinting 1, 27–38 (2015).


    Google Scholar
     

  • Prater, T. et al. 3D printing in zero G technology demonstration mission: complete experimental results and summary of related material modeling efforts. Int. J. Adv. Manuf. Technol. 101, 391–417 (2019).

    Article 
    PubMed 

    Google Scholar
     

  • Banks, M., Johnson, R., Giver, L., Bryant, G. & Guo, M. Industrial production of microbial protein products. Curr. Opin. Biotechnol. 75, 102707 (2022).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Leave a Comment