Scientists Discover Why Late-Night Eating Leads to Diabetes and Weight Gain

New research has shown for the first time that energy release may be the molecular mechanism through which our internal clocks control energy balance. These findings have wide-ranging implications, from diet to sleep loss and more.

Northwestern Medicine scientists have discovered the mechanism behind why eating late at night is linked to diabetes and weight gain. According to the CDC, 37.3 million Americans have diabetes, which represents 11.3% of the US population. An additional 96 million Americans over the age of 18 have prediabetes, representing 38.0% of the US adult population. Obesity is a common, serious, and costly disease, with obesity prevalence in the US 41.9%, according to the CDC.

The connection between mealtime, sleep, and obesity is well known but poorly understood, and research shows that overnutrition can change adipose tissue and disrupt circadian rhythms.

For the first time, new Northwestern research has shown that energy release may be the molecular mechanism through which our internal clocks control energy balance. From this understanding, the researcher also discovered that the day is the ideal time in the light environment of the Earth’s rotation when it is most optimal to dissipate energy as heat. These findings have broad implications, from diet to sleep loss, as well as how we feed patients who require long-term nutritional support.

The article, “Time-Restricted Feeding Mitigates Obesity Through Adipocyte Thermogenesis,” was published Oct. 20 in the journal Sciences.

“It’s well known, though poorly understood, that insults to the biological clock are going to be insults to metabolism,” said the study’s corresponding author, Dr. Joseph T. Bass, the Charles F. Kettering professor of medicine at the[{” attribute=””>Northwestern University Feinberg School of Medicine. He also is a Northwestern Medicine endocrinologist.

“When animals consume Western-style cafeteria diets — high fat, high carb — the clock gets scrambled,” Bass said. “The clock is sensitive to the time people eat, especially in fat tissue, and that sensitivity is thrown off by high-fat diets. We still don’t understand why that is, but what we do know is that as animals become obese, they start to eat more when they should be asleep. This research shows why that matters.”

Bass is also director of the Center for Diabetes and Metabolism and the chief of endocrinology in the department of medicine at Feinberg. Chelsea Hepler, a postdoctoral fellow in the Bass Lab, was the first author and did many of the biochemistry and genetics experiments that grounded the team’s hypothesis. Rana Gupta, now at Duke University, was also a key collaborator.

Scrambling the internal clock

In the study, mice, who are nocturnal, were fed a high-fat diet either exclusively during their inactive (light) period or during their active (dark) period. Within a week, mice fed during light hours gained more weight compared to those fed in the dark. To mitigate the effects of temperature on their findings, the scientists set the temperature to 30 degrees, where mice expend the least energy.

“We thought maybe there’s a component of energy balance where mice are expending more energy eating at specific times,” Hepler said. “That’s why they can eat the same amount of food at different times of the day and be healthier when they eat during active periods versus when they should be sleeping.”

The increase in energy expenditure led the team to look into metabolism of fat tissue to see if the same effect occurred within the endocrine organ. They found that it did, and mice with genetically enhanced thermogenesis — or heat release through fat cells — prevented weight gain and improved health.

Hepler also identified futile creatine cycling, in which creatine (a molecule that helps maintain energy) undergoes storage and release of chemical energy, within fat tissues, implying creatine may be the mechanism underlying heat release.

Findings could inform chronic care

The science is underpinned by research done by Bass and colleagues at Northwestern more than 20 years ago that found a relationship between the internal molecular clock and body weight, obesity, and metabolism in animals.

The challenge for Bass’s lab, which focuses on using genetic approaches to study physiology, has been figuring out what it all means, and finding the control mechanisms that produce the relationship. This study brings them a step closer.

The findings could inform chronic care, Bass said, especially in cases where patients have gastric feeding tubes. Patients are commonly fed at night while they sleep, when they’re releasing the least amount of energy. Rates of diabetes and obesity tend to be high for these patients, and Bass thinks this could explain why. He also wonders how the research could impact Type II Diabetes treatment. Should meal times be considered when insulin is given, for example?

Hepler will continue to research creatine metabolism. “We need to figure out how, mechanistically, the circadian clock controls creatine metabolism so that we can figure out how to boost it,” she said. “Clocks are doing a lot to metabolic health at the level of fat tissue, and we don’t know how much yet.”

Reference: “Time-restricted feeding mitigates obesity through adipocyte thermogenesis” by Chelsea Hepler, Benjamin J. Weidemann, Nathan J. Waldeck, Biliana Marcheva, Jonathan Cedernaes, Anneke K. Thorne, Yumiko Kobayashi, Rino Nozawa, Marsha V. Newman, Peng Gao, Mengle Shao, Kathryn M. Ramsey, Rana K. Gupta and Joseph Bass, 20 October 2022, Science.
DOI: 10.1126/science.abl8007